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Abnova
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Merck KGaA
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Image Search Results
Journal: mSphere
Article Title: Toxoplasma gondii AP2XII-2 Contributes to Proper Progression through S-Phase of the Cell Cycle
doi: 10.1128/mSphere.00542-20
Figure Lengend Snippet: Immunofluorescence assay of AP2IX-4 and AP2XII-2 during cell cycle. (A) RHΔ ku80 (RH) parasites expressing endogenously tagged AP2XII-2 MYC were probed with antibodies to c-MYC (green) and IMC3 (red), the latter of which helps detect budding daughter parasites. AP2XII-2 MYC was detectable in the S and S/M phases but disappeared before the onset of cytokinesis (C). DAPI (blue) was used as a nuclear stain. The four rows show parasites in different stages of the cell cycle. Scale bar = 2 μm. (B) To more closely examine the expression of AP2XII-2 MYC during cell cycle, an IFA was performed using antibodies to c-MYC (green) and Centrin-1 (red). Parasites in G 1 contain single centrosomes, whereas those in S-phase are duplicated. Arrows indicate centrosomes. Scale bar = 2 μm. (C) Triple-labeled IFA to determine cell cycle expression patterns for each AP2 factor. AP2XII-2 MYC (green) and AP2IX-4 HA (red) colocalized in S-phase. IMC3 (yellow) and larger nuclei (DAPI, blue) identify parasites in S-phase. Scale bar = 2 μm.
Article Snippet: Primary antibody dilutions were used as follows: rabbit anti-HA (Cell Signaling) 1:1,000, rat anti-HA (Roche) 1:1,000, mouse anti-MYC (Cell Signaling) 1:1,000,
Techniques: Immunofluorescence, Expressing, Staining, Labeling
Journal: mSphere
Article Title: Toxoplasma gondii AP2XII-2 Contributes to Proper Progression through S-Phase of the Cell Cycle
doi: 10.1128/mSphere.00542-20
Figure Lengend Snippet: AP2XII-2 depletion results in a delay in S-phase of cell cycle. (A) A total of 100 parasite vacuoles in 10 random fields were counted for single or duplicated centrosomes in 3 biological replicates. Tukey's multiple-comparison test was performed with two-way ANOVA. Error bars represent standard deviations of the means; ****, P < 0.0001; ns = not significant. (B) Representative IFAs of AP2XII-2 AID-HA parasites with or without IAA showing single (S) and duplicated (D) centrosomes as stained by anti-Centrin-1 (green). Scale bar =10 μm. (C) The number of parasites per vacuole was counted at each indicated time point for 100 randomly chosen vacuoles to calculate doubling time of AP2XII-2 AID-HA parasites with or without IAA. Error bars represent mean ± SD ( n = 3). The exponential growth equation was used to calculate the doubling time in GraphPad Prism software as described previously . (D) The percentage of AP2XII-2 AID-HA parasites (with [+] or without [-] IAA) in various stages of the cell cycle was determined as described in the text. Doubling times (DT) in hours determined as described for panel C are also shown.
Article Snippet: Primary antibody dilutions were used as follows: rabbit anti-HA (Cell Signaling) 1:1,000, rat anti-HA (Roche) 1:1,000, mouse anti-MYC (Cell Signaling) 1:1,000,
Techniques: Staining, Software